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To assess the dependence of PDAC cells on extracellular fatty acids we employed delipidated serum or RNAi mediated suppression of ACSL3 to inhibit the activation and cellular retention of extracellular fatty acids followed by cell proliferation assays, qPCR, apoptosis assays, immunoblots and fluorescence microscopy experiments torsemide vs lasix At both points analyses were performed to assess the rate of tumor cell proliferation
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