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J Affect Disord 2011; 129 327 331 Crossref, Google Scholar tamoxifen breast cancer We subsequently cocultured the polarized macrophages with suboptimally activated T cells CD3 CD28 and IL 2 isolated from spleens of non tumor bearing mice for 72 hours, following which they were treated with protein transport inhibitors monensin and brefeldin for 6 hours to prevent the release of cytokines and chemokines